Researchers started testing its effects on tissues relevant to reproduction, including ovarian cells, endometrium, and testes
They monitor for improved sleep architecture (via wearable/tracking), morning recovery feeling, and reduction in stress hormones or improved hormone balance (if they have access to such measures)
Neuron 5: 841846
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Notably, in their HEK293 cell line, Ser363 was constitutively phosphorylated but showed no increase with opioid agonists, and Thr370 could be phosphorylated by stimulation of PKC activity (see below) in addition to DAMGO
These microbial shifts trigger cascading gutbrain disruption by impairing neurotransmitter signaling, suppressing SCFA production, weakening epithelial barrier integrity, and activating neuroimmune pathways, ultimately resulting in anxiety- and depression-like behaviors in rodent models (Tejkalov et al., 2023
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